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The detection of somatic point mutations is a key component of cancer genomic research, which has been rapidly developing since next-generation sequencing (NGS) technology revealed its potential for describing genetic alterations in cancer. We present MuSE, a novel approach to mutation calling based on the F81 Markov substitution model for molecular evolution nike air max 1 black leopard buy silver
, which models the evolution of the reference allele to the allelic composition of the matched tumor and normal tissue at each genomic locus. To improve overall accuracy, we further adopt a sample-specific error model to identify cutoffs, reflecting the variation in tumor heterogeneity among samples.

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After downloading the source file, for Unix-like operating systems please type the following commands sequentially in the command line to generate the executable:

For Windows, please install Cygwin ( buy nike free run online uke
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MuSE is comprised of two steps, which requires

The first step, ‘MuSE call’, takes as input (1) and (2). The BAM files require aligning all the sequence reads against the reference genome using the Burrows-Wheeler alignment tool (BWA), with either the backtrack or the maximal exact matches (MEM) algorithm [2] . In addition, the BAM files need to be processed by following the Genome Analysis Toolkit (GATK) Best Practices [3-5] that include marking duplicates, realigning the paired tumor-normal BAMs jointly and recalibrating base quality scores.

To speed up ‘MuSE call’, we recommend splitting the WGS data into small blocks (<50Mb) by using the provided option either ‘-r’ or ‘-l’, and concatenating all the output files by the Linux command CAT.

The second step, ‘MuSE sump’, takes as input the output file from ‘MuSE call’ and (3). We provide two options for building the sample-specific error model. One is applicable to WES data (option ‘-E’), and the other to WGS data (option ‘-G’).

The following commands briefly illustrate how to use MuSE. As to the preparation of BAM files, please refer to the first part, PRE-PROCESSING, of the Genome Analysis Toolkit (GATK) Best Practices ( nike mens air max dynasty running shoes
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Welcome to the Juror Web Portal.

ADDRESS: 50 Maryland Avenue, North Tower 2nd Floor, Rm 2100, Rockville, MD 20850 Directions P Juror Parking

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PHONE HOURS: Monday through Friday 10:30am - 4:00pm

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NOTE: The Circuit Court opens at 7:30 a.m. Please note that you will need at least 15-20 minutes to find parking, get through security, and check in at the jury lounge.

For questions regarding Jury Duty, please call 240-777-9090. Please have your badge ID number available when you call.

NOTE: The following information is NOT for Grand Jurors! If you are a Grand Juror, please follow the instructions on your letter and summons. More information is located on the About Grand Jury Service page.

The following information is NOT for Grand Jurors!

Sent to all prospective jurors and used to determine whether an individual is qualified

Map to guide you to the Circuit Court by car, rail, bus, or foot.

Directions to parking around the Courthouse.

What trial jurors and Grand Jurors can expect when they arrive

...in the courthouse and in the courtroom

WIFI access; map to local eateries and shops in Rockville Town Center

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A Montgomery County juror must be at least 18 years of age, a citizen of the United States, a resident of Montgomery County, and be able to communicate in English.

Any citizen selected for a jury trial serves for the duration of one trial, regardless of how long that trial may be. Montgomery County participates in the "one day, one trial" process. If you are not assigned to a trial by the end of the day, your service is complete. The judge reserves the right to keep a panel of jurors longer than one day if the jury selection process has not finished.

The average length of our trials is 2-3 days. However, some trials may be longer. You will be told the length of the trial upon arrival in the courtroom.

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